Infection with Porphyromonas gingivalis exacerbates endothelial injury in obese mice.

Infection with Porphyromonas gingivalis exacerbates endothelial injury in obese mice.

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BACKGROUND:A number of studies have perdieme lingerie revealed a link between chronic periodontitis and cardiovascular disease in obese patients.However, there is little information about the influence of periodontitis-associated bacteria, Porphyromonas gingivalis (Pg), on pathogenesis of atherosclerosis in obesity.METHODS:In vivo experiment: C57BL/6J mice were fed with a high-fat diet (HFD) or normal chow diet (CD), as a control.Pg was infected from the pulp chamber.

At 6 weeks post-infection, histological and immunohistochemical analysis of aortal tissues was performed.In vitro experiment: hTERT-immortalized human umbilical vein endothelial cells (HuhT1) were used to assess the effect of Pg/Pg-LPS on free fatty acid (FFA) induced endothelial cells apoptosis and regulation of cytokine gene expression.RESULTS:Weaker staining of CD31 and increased numbers of TUNEL positive cells in aortal tissue of HFD mice indicated endothelial injury.Pg infection exacerbated the endothelial injury.

Immunohistochemically, Pg was detected deep in the smooth muscle of the aorta, and the number of Pg cells in the aortal wall was higher in HFD mice than in CD mice.Moreover, in vitro, FFA treatment induced apoptosis in HuhT1 cells and exposure to Pg-LPS increased this effect.In addition, Pg and Pg-LPS both attenuated cytokine production in HuhT1 cells poison open up and say ahh t shirt stimulated by palmitate.CONCLUSIONS:Dental infection of Pg may contribute to pathogenesis of atherosclerosis by accelerating FFA-induced endothelial injury.